Semax Quick Reference (10 mg vial)
Research context: mechanisms, human and preclinical evidence, limitations and precautions are in How this works and References. The calculator (Spanish) converts any other volume.
Semax Dosage Chart (10 mg vial)
No published clinical trial establishes a dosing regimen for Semax. Any figure circulating as a human protocol does not come from an indexed trial. This site does not publish doses without a verifiable source.
U-100 Conversion Table (2 mL = 5 mg/mL)
| U-100 units | Volume | Contains | Yield per vial |
|---|---|---|---|
| 2 | 0.02 mL | 100 mcg | 100 injections |
| 5 | 0.05 mL | 250 mcg | 40 injections |
| 10 | 0.10 mL | 500 mcg | 20 injections |
| 20 | 0.20 mL | 1 mg (1000 mcg) | 10 injections |
| 30 | 0.30 mL | 1.5 mg (1500 mcg) | 6 injections |
| 50 | 0.50 mL | 2.5 mg (2500 mcg) | 4 injections |
| 100 | 1.00 mL | 5 mg (5000 mcg) | 2 injections |
Reconstitution Steps
- Draw 2 mL of bacteriostatic water with a sterile syringe.1
- Inject slowly down the vial wall; avoid shaking to prevent foaming.2
- Gently swirl or roll until fully dissolved (clear solution).3
- Label with the reconstitution date and refrigerate at 2–8 °C, protected from light; use within 4 weeks.4
Supplies Needed
Per 10 mg vial: 2 mL of bacteriostatic water, one U-100 syringe per injection (plus one to reconstitute) and two alcohol swabs per injection.
Semax 10mgView in store
Bacteriostatic water 3 mLView in storeStore links may earn a commission. A supplier page is not scientific evidence or proof of suitability for human use.
Semax Vial and Research Context
- Reconstitute: add 2 mL of bacteriostatic water to produce 5 mg/mL.
- Easy measuring: 1 U-100 unit = 0.01 mL = 50 mcg.
- Vial size: the 10 mg label is total nominal content, not a per-injection amount.
Storage Instructions
- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.
More detail in the storage guide (Spanish).
Important Notes
- Research only: the referenced compounds are sold for laboratory research. Nothing here is medical advice or an administration schedule.
- Above 100 units: a 1 mL U-100 syringe cannot hold them; split across syringes or reconstitute with less volume.
- Accuracy: concentration depends on the actual volume added; measure the diluent with a syringe, not by eye.
- Verification: confirm vial contents against the batch certificate of analysis before calculating.
How This Works
- Increased BDNF and NGF: Semax stimulates the brain's expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), key factors for synaptic plasticity.
- Neurotransmitter Modulation increases dopaminergic and serotonergic tone in specific regions, without causing massive release
- Inhibition of enkephalins and endorphins: protect these endogenous opioids from enzymatic degradation
- Neuroprotective action reduce neuronal damage in models of cerebral ischemia
- Melanocortin receptor activation contributes to the regulation of alertness and attention
Research Applications
- Post-ischemic stroke recovery (clinical use approved in Russia)
- Memory and learning enhancement in healthy adults
- Attention deficit disorder (ADHD)
- Cognitive fatigue and burnout
- Post-traumatic brain injury recovery
- Research in mild cognitive impairment and early dementia
- Performance improvement in demanding cognitive tasks
Precautions Described in the Literature
Excellent safety profile documented in decades of Russian clinical use. Rare adverse events: local nasal irritation with prolonged intranasal use, mild headache, insomnia if administered late in the day.
Avoid in patients with bipolar disorder or active psychosis due to its dopaminergic action. Do not combine with MAOIs or dopamine reuptake inhibitors without supervision.
Studied Combinations
- Cognition + emotional stability Semax + Selank (classic Russian combination)
- Neurological Recovery Semax + NAD+ for cerebral mitochondrial support
- Integral burnout: Semax + Selank + DSIP for focus, calm, and sleep
Injection Technique in Research
- Route in the studies: subcutaneous, in abdominal or thigh adipose tissue, rotating the site.
- Syringe: 1 mL U-100 (100 units); read in units, not mL.
- Asepsis: alcohol swab on the stopper and the site; a new syringe per injection.
- Drawing: purge air, draw the units from the chart and verify before withdrawing the needle from the vial.
Full guide: U-100 syringes and reading units (Spanish).
Research Note
All content on this page is technical and educational. It describes how a vial is reconstituted, how a syringe is read and which amounts the cited sources describe. It does not replace the supervision of a qualified researcher or the judgment of a health professional, and it is not intended to diagnose, treat, cure or prevent any disease.
References
- Asmarin IP, Nezavibatko VN, Myasoedov NF, et al. A nootropic adrenocorticotropin analog 4-10-Semax: 15 years experience in development and study. J Higher Nervous Activity I P Pavlov. 1997;47(2):420-430.
- Dolotov OV, Karpenko EA, Inozemtseva LS, et al. Semax, an analog of adrenocorticotropin (4-10), specifically binds and increases brain-derived neurotrophic factor protein levels in the rat basal forebrain. J Neurochem. 2006;97(Suppl 1):82-86.
- Shadrina MI, Dolotov OV, Grivennikov IA, et al. Rapid induction of neurotrophin mRNAs in rat glial cell cultures by Semax, an adrenocorticotropic hormone analog. Neurosci Lett. 2001;308(2):115-118.