# TB-500 dosage & reconstitution (10 mg vial)

TB-500 dosage: calculations for a 10 mg vial use the same amounts described in the literature. Adding 2 mL of bacteriostatic water produces 5 mg/mL, so each U-100 unit contains 50 mcg.

- **Vial contents:** 10 mg TB-500
- **Final volume:** 2 mL
- **Concentration:** 5 mg/mL
- **One U-100 unit:** 50 mcg in 0.01 mL

## Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing

### Standard / Gradual Approach (2 mL = ~5 mg/mL)

| Week / phase | Daily dose | Units per injection (mL) |
|---|---|---|
| Weeks 1–2 | 500 mcg | 10 units (0.10 mL) |
| Weeks 3–4 | 600 mcg | 12 units (0.12 mL) |
| Weeks 5–8 | 750 mcg | 15 units (0.15 mL) |
| Weeks 9–12 | 1 mg (1000 mcg) | 20 units (0.20 mL) |

Frequency: Once daily, subcutaneously, at a consistent time.

### Reconstitution Steps

1. Draw 2 mL of bacteriostatic water with a sterile syringe.
2. Inject slowly down the vial wall; avoid shaking to prevent foaming.
3. Gently swirl or roll until fully dissolved (clear solution).
4. Label with the reconstitution date and refrigerate at 2–8 °C, protected from light; use within 4 weeks.

Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

## U-100 Conversion Table

| U-100 units | Volume | Contains | Yield per vial |
|---|---|---|---|
| 2 | 0.02 mL | 100 mcg | 100 injections |
| 5 | 0.05 mL | 250 mcg | 40 injections |
| 10 | 0.10 mL | 500 mcg | 20 injections |
| 20 | 0.20 mL | 1 mg (1000 mcg) | 10 injections |
| 30 | 0.30 mL | 1.5 mg (1500 mcg) | 6 injections |
| 50 | 0.50 mL | 2.5 mg (2500 mcg) | 4 injections |
| 100 | 1.00 mL | 5 mg (5000 mcg) | 2 injections |

## Supplies Needed

**TB-500 vials (10 mg each)**
- Standard / Gradual Approach, 12 weeks: Minimum 7 vials (64.4 mg required).
- Standard / Gradual Approach, 24 weeks: Minimum 15 vials (148.4 mg required).

**U-100 syringes (1 mL)**
- Standard / Gradual Approach, 12 weeks: 84 new syringes for 84 injections.
- Standard / Gradual Approach, 24 weeks: 168 new syringes for 168 injections.

**Bacteriostatic water**
- Standard / Gradual Approach, 12 weeks: 14 mL total (2 mL per vial) → 5 × 3 mL vials.
- Standard / Gradual Approach, 24 weeks: 30 mL total (2 mL per vial) → 10 × 3 mL vials.

**Alcohol swabs**
- Standard / Gradual Approach, 12 weeks: 168 minimum; one box of 100 does not cover the calculation.
- Standard / Gradual Approach, 24 weeks: 336 minimum; one box of 100 does not cover the calculation.

## Storage Instructions

- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.

## How This Works

TB-500 exerts its effects primarily through regulation of G-actin (globular) and F-actin (filamentous): Secuestro de actina G: maintains a pool of monomeric actin available for the dynamic formation of filaments during cell migration Promoción de angiogénesis: estimula proliferación y migración de células endoteliales, aumentando densidad vascular en tejidos dañados Modulación inflamatoria: reduce expresión de citoquinas pro-inflamatorias y promueve resolución de inflamación Estimulación de células madre: promueve diferenciación de células progenitoras locales

## References

1. Goldstein AL, Hannappel E, Kleinman HK. Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421-429.
2. Crockford D. Development of thymosin beta4 for treatment of patients with ischemic heart disease. Ann N Y Acad Sci. 2007;1112:385-395.
3. Sosne G, Qiu P, Goldstein AL, Wheater M. Biological activities of thymosin beta4 defined by active sites in short peptide sequences. FASEB J. 2010;24(7):2144-2151.

---
Source: https://protocolopeptidos.com/en/compuestos/tb-500-10mg · Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.