# SS-31 dosage & reconstitution (50 mg vial)

SS-31 dosage: calculations for a 50 mg vial use the same amounts described in the literature. Adding 2 mL of bacteriostatic water produces 25 mg/mL, so each U-100 unit contains 250 mcg.

- **Vial contents:** 50 mg SS-31
- **Final volume:** 2 mL
- **Concentration:** 25 mg/mL
- **One U-100 unit:** 250 mcg in 0.01 mL

## Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing

### Standard / Gradual Approach (2 mL = ~25 mg/mL)

| Week / phase | Daily dose | Units per injection (mL) |
|---|---|---|
| Weeks 1–2 | 5 mg (5000 mcg) | 20 units (0.20 mL) |
| Weeks 3–8 | 10 mg (10000 mcg) | 40 units (0.40 mL) |

Frequency: Once daily, subcutaneously, at a consistent time.

### Reconstitution Steps

1. Draw 2 mL of bacteriostatic water with a sterile syringe.
2. Inject slowly down the vial wall; avoid shaking to prevent foaming.
3. Gently swirl or roll until fully dissolved (clear solution).
4. Label with the reconstitution date and refrigerate at 2–8 °C, protected from light; use within 4 weeks.

### Advanced / Aggressive Approach (2 mL = ~25 mg/mL)

| Week / phase | Daily dose | Units per injection (mL) |
|---|---|---|
| Weeks 1–2 | 5 mg (5000 mcg) | 20 units (0.20 mL) |
| Weeks 3–4 | 10 mg (10000 mcg) | 40 units (0.40 mL) |
| Weeks 5–8 | 15 mg (15000 mcg) | 60 units (0.60 mL) |
| Optional Weeks 9–12 | 20 mg (20000 mcg) | 80 units (0.80 mL) |

Frequency: Once daily, subcutaneously, at a consistent time.

> The advanced approach (15–20 mg/day) is based on short-term clinical-trial protocols in severe mitochondrial disease and is described for educational purposes only.

Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

## U-100 Conversion Table

| U-100 units | Volume | Contains | Yield per vial |
|---|---|---|---|
| 2 | 0.02 mL | 500 mcg | 100 injections |
| 5 | 0.05 mL | 1.25 mg (1250 mcg) | 40 injections |
| 10 | 0.10 mL | 2.5 mg (2500 mcg) | 20 injections |
| 20 | 0.20 mL | 5 mg (5000 mcg) | 10 injections |
| 30 | 0.30 mL | 7.5 mg (7500 mcg) | 6 injections |
| 50 | 0.50 mL | 12.5 mg (12500 mcg) | 4 injections |
| 100 | 1.00 mL | 25 mg (25000 mcg) | 2 injections |

## Supplies Needed

**SS-31 vials (50 mg each)**
- Standard / Gradual Approach, 12 weeks: Minimum 16 vials (770 mg required).
- Standard / Gradual Approach, 24 weeks: Minimum 33 vials (1610 mg required).
- Advanced / Aggressive Approach, 12 weeks: Minimum 24 vials (1190 mg required).
- Advanced / Aggressive Approach, 24 weeks: Minimum 58 vials (2870 mg required).

**U-100 syringes (1 mL)**
- Standard / Gradual Approach, 12 weeks: 84 new syringes for 84 injections.
- Standard / Gradual Approach, 24 weeks: 168 new syringes for 168 injections.
- Advanced / Aggressive Approach, 12 weeks: 84 new syringes for 84 injections.
- Advanced / Aggressive Approach, 24 weeks: 168 new syringes for 168 injections.

**Bacteriostatic water**
- Standard / Gradual Approach, 12 weeks: 32 mL total (2 mL per vial) → 11 × 3 mL vials.
- Standard / Gradual Approach, 24 weeks: 66 mL total (2 mL per vial) → 22 × 3 mL vials.
- Advanced / Aggressive Approach, 12 weeks: 48 mL total (2 mL per vial) → 16 × 3 mL vials.
- Advanced / Aggressive Approach, 24 weeks: 116 mL total (2 mL per vial) → 39 × 3 mL vials.

**Alcohol swabs**
- Standard / Gradual Approach, 12 weeks: 168 minimum; one box of 100 does not cover the calculation.
- Standard / Gradual Approach, 24 weeks: 336 minimum; one box of 100 does not cover the calculation.
- Advanced / Aggressive Approach, 12 weeks: 168 minimum; one box of 100 does not cover the calculation.
- Advanced / Aggressive Approach, 24 weeks: 336 minimum; one box of 100 does not cover the calculation.

## Storage Instructions

- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.

## References

1. Programa clínico de elamipretida (Stealth BioTherapeutics)

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Source: https://protocolopeptidos.com/en/compuestos/ss-31-50mg · Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.