SS-31 dosage & reconstitution (50 mg vial)

Vial contents50 mg SS-31

Final volume2 mL

Concentration25 mg/mL

One U-100 unit250 mcg in 0.01 mL

Research context: mechanisms, human and preclinical evidence, limitations and precautions are in How this works and References. The calculator (Spanish) converts any other volume.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (2 mL = ~25 mg/mL)

Frequency: Once daily, subcutaneously, at a consistent time.

Reconstitution Steps

1. Draw 2 mL of bacteriostatic water with a sterile syringe.1
2. Inject slowly down the vial wall; avoid shaking to prevent foaming.2
3. Gently swirl or roll until fully dissolved (clear solution).3
4. Label with the reconstitution date and refrigerate at 2–8 °C, protected from light; use within 4 weeks.4

Advanced / Aggressive Approach (2 mL = ~25 mg/mL)

Frequency: Once daily, subcutaneously, at a consistent time.

Note: The advanced approach (15–20 mg/day) is based on short-term clinical-trial protocols in severe mitochondrial disease and is described for educational purposes only.

Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

U-100 Conversion Table (2 mL = 25 mg/mL)

This section describes which dose a study or reference source used and what it measured. It is not a recommendation or an administration schedule. A trial dose belongs to its population, duration and supervision and does not transfer to anyone outside that context.

Straightforward 12- and 24-week planning for a 50 mg vial at 25 mg/mL.

Sharps container: one appropriately sized. Stability planning: totals are minimum arithmetic quantities; follow the product documentation for sterility, storage, handling loss and discard timing.

SS-31 50mgView in store

U-100 syringes (1 mL)1 mL, fine needle

Bacteriostatic water 3 mLView in store

Alcohol swabs70 % isopropyl

Store links may earn a commission. A supplier page is not scientific evidence or proof of suitability for human use.

• Reconstitute: add 2 mL of bacteriostatic water to produce 25 mg/mL.
• Frequency in the source: once daily, subcutaneously.
• Described range: schedules run from 5 mg (5000 mcg) to 20 mg (20000 mcg) per injection.
• Easy measuring: 1 U-100 unit = 0.01 mL = 250 mcg.
• Vial size: the 50 mg label is total nominal content, not a per-injection amount.
• Vial coverage: at 5 mg (5000 mcg) per injection one vial supplies 10 injections; at 20 mg (20000 mcg) it supplies 2. These are mass calculations, not storage periods.

Quick reference for the schedules shared by the cited literature.

• Frequency: once daily.
• Concentration: 50 mg in 2 mL = 25 mg/mL.
• Unit conversion: 1 U-100 unit = 250 mcg.

The tables keep each approach separate.

• Standard / Gradual Approach: 5 mg (5000 mcg), 10 mg (10000 mcg), in phases (once daily).
• Advanced / Aggressive Approach: 5 mg (5000 mcg), 10 mg (10000 mcg), 15 mg (15000 mcg), 20 mg (20000 mcg), in phases (once daily).
• Measurement: use the units and mL shown beside each amount.

• Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
• Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
• Handling: do not shake; avoid temperature cycling; label with date and concentration.
• Discard: cloudy solutions, particulates or expired preparations are discarded.

More detail in the storage guide (Spanish).

• Research only: the referenced compounds are sold for laboratory research. Nothing here is medical advice or an administration schedule.
• Evidence: chart amounts come from published trials or approved prescribing information, cited in References.
• Above 100 units: a 1 mL U-100 syringe cannot hold them; split across syringes or reconstitute with less volume.
• Accuracy: concentration depends on the actual volume added; measure the diluent with a syringe, not by eye.
• Verification: confirm vial contents against the batch certificate of analysis before calculating.

• Route in the studies: subcutaneous, in abdominal or thigh adipose tissue, rotating the site.
• Syringe: 1 mL U-100 (100 units); read in units, not mL.
• Asepsis: alcohol swab on the stopper and the site; a new syringe per injection.
• Drawing: purge air, draw the units from the chart and verify before withdrawing the needle from the vial.

Full guide: U-100 syringes and reading units (Spanish).

All content on this page is technical and educational. It describes how a vial is reconstituted, how a syringe is read and which amounts the cited sources describe. It does not replace the supervision of a qualified researcher or the judgment of a health professional, and it is not intended to diagnose, treat, cure or prevent any disease.

1. Programa clínico de elamipretida (Stealth BioTherapeutics)

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