# Semax dosage & reconstitution (10 mg vial)

Semax dosage: calculations for a 10 mg vial use the same amounts described in the literature. Adding 2 mL of bacteriostatic water produces 5 mg/mL, so each U-100 unit contains 50 mcg.

- **Vial contents:** 10 mg Semax
- **Final volume:** 2 mL
- **Concentration:** 5 mg/mL
- **One U-100 unit:** 50 mcg in 0.01 mL

No published clinical trial establishes a dosing regimen for Semax. Any figure circulating as a human protocol does not come from an indexed trial. This site does not publish doses without a verifiable source.

## U-100 Conversion Table

| U-100 units | Volume | Contains | Yield per vial |
|---|---|---|---|
| 2 | 0.02 mL | 100 mcg | 100 injections |
| 5 | 0.05 mL | 250 mcg | 40 injections |
| 10 | 0.10 mL | 500 mcg | 20 injections |
| 20 | 0.20 mL | 1 mg (1000 mcg) | 10 injections |
| 30 | 0.30 mL | 1.5 mg (1500 mcg) | 6 injections |
| 50 | 0.50 mL | 2.5 mg (2500 mcg) | 4 injections |
| 100 | 1.00 mL | 5 mg (5000 mcg) | 2 injections |

## Storage Instructions

- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.

## How This Works

Increased BDNF and NGF: Semax stimulates the brain's expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), key factors for synaptic plasticity. Neurotransmitter Modulation increases dopaminergic and serotonergic tone in specific regions, without causing massive release Inhibition of enkephalins and endorphins: protect these endogenous opioids from enzymatic degradation Neuroprotective action reduce neuronal damage in models of cerebral ischemia Melanocortin receptor activation contributes to the regulation of alertness and attention

## References

1. Asmarin IP, Nezavibatko VN, Myasoedov NF, et al. A nootropic adrenocorticotropin analog 4-10-Semax: 15 years experience in development and study. J Higher Nervous Activity I P Pavlov. 1997;47(2):420-430.
2. Dolotov OV, Karpenko EA, Inozemtseva LS, et al. Semax, an analog of adrenocorticotropin (4-10), specifically binds and increases brain-derived neurotrophic factor protein levels in the rat basal forebrain. J Neurochem. 2006;97(Suppl 1):82-86.
3. Shadrina MI, Dolotov OV, Grivennikov IA, et al. Rapid induction of neurotrophin mRNAs in rat glial cell cultures by Semax, an adrenocorticotropic hormone analog. Neurosci Lett. 2001;308(2):115-118.

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Source: https://protocolopeptidos.com/en/compuestos/semax-10mg · Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.