# HCG 5000iu: reference sheet

HCG 5000iu is a laboratory supply: it is not reconstituted. This sheet covers its use as a diluent or support, storage and available sizes.

- **Vial contents:** 5000 iu
- **Storage:** 15–25 °C, protected from light
- **Use:** laboratory diluent / support

No published clinical trial establishes a dosing regimen for HCG. Any figure circulating as a human protocol does not come from an indexed trial. This site does not publish doses without a verifiable source.

## Storage Instructions

- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.

## How This Works

Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone (MW: 36,700 Da) composed of two non-covalently linked subunits (α: 92 aa, β: 145 aa). Naturally produced by the placental syncytiotrophoblast during pregnancy, hCG exhibits biological activity analogous to pituitary LH (luteinizing hormone) with greater potency and extended half-life. Molecular Structure: Alpha subunit Identical to LH, FSH, TSH (common) Beta subunit: Specific hCG (confers biological specificity) Glycosylation: 30% molecular weight (4 N-glycosylation sites, 4 O-glycosylation) Half-life: 24-36 hours (vs 20 min LH, due to hyperglycosylation) Mechanisms of Action: LH/hCG Receptor (LHCGR) Activation: Receptor: GPCR (7-transmembrane) on testicular Leydig cells, ovarian theca Transduction: Gs protein coupling → adenylate cyclase → ↑ cAMP → PKA Cascade: StAR (steroidogenic acute regulatory protein) phosphorylation Result: mitochondrial cholesterol transport → steroidogenesis Testicular Steroidogenesis: Leydig cells: testosterone synthesis stimulation Key enzymes: CYP11A1 (side-chain cleavage), 3β-HSD, 17β-HSD Testosterone production: 200-400% increase over baseline levels Paracrine effects: spermatogenesis…

## Research FAQ

**Q: hCG + FSH combination protocol for spermatogenesis?** Standard protocol for hypogonadotropic hypogonadism:
**LH/hCG receptor desensitization?** Possible with high doses or excessive frequency:
**Main side effects research?** Dose-dependent effects:

## References

1. Protocolo de estimulación con hCG en práctica endocrinológica

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Source: https://protocolopeptidos.com/en/compuestos/hcg-5000iu · Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.