# GLOW dosage & reconstitution (70 mg vial)

GLOW dosage: calculations for a 70 mg vial use the same amounts described in the literature. Adding 3 mL of bacteriostatic water produces 23.33 mg/mL, so each U-100 unit contains 233 mcg.

- **Vial contents:** 70 mg GLOW
- **Final volume:** 3 mL
- **Concentration:** 23.33 mg/mL
- **One U-100 unit:** 233 mcg in 0.01 mL

## Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing

### Standard Approach (3 mL = ~23.33 mg/mL)

| Week / phase | Daily dose | Units per injection (mL) |
|---|---|---|
| Weeks 1–4 | 2.33 mg (2330 mcg) | 10 units (0.10 mL) |

Frequency: Once daily, subcutaneously, at a consistent time.

### Reconstitution Steps

1. Draw 3 mL of bacteriostatic water with a sterile syringe.
2. Inject slowly down the vial wall; avoid shaking to prevent foaming.
3. Gently swirl or roll until fully dissolved (clear solution).
4. Label with the reconstitution date and refrigerate at 2–8 °C, protected from light; use within 4 weeks.

Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

## U-100 Conversion Table

| U-100 units | Volume | Contains | Yield per vial |
|---|---|---|---|
| 2 | 0.02 mL | 466.667 mcg | 150 injections |
| 5 | 0.05 mL | 1.16667 mg (1167 mcg) | 60 injections |
| 10 | 0.10 mL | 2.33333 mg (2333 mcg) | 30 injections |
| 20 | 0.20 mL | 4.66667 mg (4667 mcg) | 15 injections |
| 30 | 0.30 mL | 7 mg (7000 mcg) | 10 injections |
| 50 | 0.50 mL | 11.6667 mg (11667 mcg) | 6 injections |
| 100 | 1.00 mL | 23.3333 mg (23333 mcg) | 3 injections |

## Supplies Needed

**GLOW vials (70 mg each)**
- Standard Approach, 12 weeks: Minimum 3 vials (195.72 mg required).
- Standard Approach, 24 weeks: Minimum 6 vials (391.44 mg required).

**U-100 syringes (1 mL)**
- Standard Approach, 12 weeks: 84 new syringes for 84 injections.
- Standard Approach, 24 weeks: 168 new syringes for 168 injections.

**Bacteriostatic water**
- Standard Approach, 12 weeks: 9 mL total (3 mL per vial) → 3 × 3 mL vials.
- Standard Approach, 24 weeks: 18 mL total (3 mL per vial) → 6 × 3 mL vials.

**Alcohol swabs**
- Standard Approach, 12 weeks: 168 minimum; one box of 100 does not cover the calculation.
- Standard Approach, 24 weeks: 336 minimum; one box of 100 does not cover the calculation.

## Storage Instructions

- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.

## How This Works

GLOW is a research tri-peptide blend in a single 70 mg lyophilized vial that combines two tissue-repair peptides (BPC-157 and TB-500) with a copper tripeptide for dermal remodeling (GHK-Cu). The literature describes complementary pathways of angiogenesis, cell migration, and extracellular matrix synthesis in experimental models. Blend composition (70 mg lyophilized per vial): BPC-157 (10 mg): pentadecapeptide derived from a gastric juice protective sequence. Preclinical literature documents upregulation of VEGFR2 (angiogenesis) and repair of tendon, ligament, and mucosa in murine models. Vasireddi N et al., Am J Sports Med 2025 (PMID 40756949) review 36 studies on the modulation of tissue repair pathways. TB-500 (10 mg): Synthetic fragment (LKKTETQ) of Thymosin Beta-4. Literature documents cell migration, angiogenesis via AC-SDKP, and cytokine modulation. Crockford D et al., Ann N Y Acad Sci 2010 (PMID 20955331) y Sosne G et al., Exp Eye Res 2002 (PMID 11819014) in healing models. GHK-Cu (50 mg): Gly-His-Lys tripeptide complexed with copper (II). Literature describes synthesis of type I/III collagen, extracellular matrix modulation, antioxidant activity, and suppression of TGF-β1.…

---
Source: https://protocolopeptidos.com/en/compuestos/glow-70mg · Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.