# DSIP dosage & reconstitution (5 mg vial)

DSIP dosage: calculations for a 5 mg vial use the same amounts described in the literature. Adding 1 mL of bacteriostatic water produces 5 mg/mL, so each U-100 unit contains 50 mcg.

- **Vial contents:** 5 mg DSIP
- **Final volume:** 1 mL
- **Concentration:** 5 mg/mL
- **One U-100 unit:** 50 mcg in 0.01 mL

## Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing

### Standard / Gradual Approach (1 mL = ~5 mg/mL)

| Week / phase | Daily dose | Units per injection (mL) |
|---|---|---|
| Week 1 | 100 mcg | 2 units (0.02 mL) |
| Week 2 | 150 mcg | 3 units (0.03 mL) |
| Week 3 | 200 mcg | 4 units (0.04 mL) |
| Weeks 4–8 | 300 mcg | 6 units (0.06 mL) |

Frequency: Once daily, subcutaneously, at a consistent time.

### Reconstitution Steps

1. Draw 1 mL of bacteriostatic water with a sterile syringe.
2. Inject slowly down the vial wall; avoid shaking to prevent foaming.
3. Gently swirl or roll until fully dissolved (clear solution).
4. Label with the reconstitution date and refrigerate at 2–8 °C, protected from light; use within 4 weeks.

### Advanced / Extended Approach (1 mL = ~5 mg/mL)

| Week / phase | Daily dose | Units per injection (mL) |
|---|---|---|
| Week 5 | 400 mcg | 8 units (0.08 mL) |
| Weeks 6–8+ | 500 mcg | 10 units (0.10 mL) |

Frequency: Once daily, subcutaneously, at a consistent time.

> Amounts above 300 mcg come from anecdotal experience; formal human studies mainly used low body-weight-based doses.

Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

## U-100 Conversion Table

| U-100 units | Volume | Contains | Yield per vial |
|---|---|---|---|
| 2 | 0.02 mL | 100 mcg | 50 injections |
| 5 | 0.05 mL | 250 mcg | 20 injections |
| 10 | 0.10 mL | 500 mcg | 10 injections |
| 20 | 0.20 mL | 1 mg (1000 mcg) | 5 injections |
| 30 | 0.30 mL | 1.5 mg (1500 mcg) | 3 injections |
| 50 | 0.50 mL | 2.5 mg (2500 mcg) | 2 injections |
| 100 | 1.00 mL | 5 mg (5000 mcg) | 1 injection |

## Supplies Needed

**DSIP vials (5 mg each)**
- Standard / Gradual Approach, 12 weeks: Minimum 5 vials (22.05 mg required).
- Standard / Gradual Approach, 24 weeks: Minimum 10 vials (47.25 mg required).

**U-100 syringes (1 mL)**
- Standard / Gradual Approach, 12 weeks: 84 new syringes for 84 injections.
- Standard / Gradual Approach, 24 weeks: 168 new syringes for 168 injections.

**Bacteriostatic water**
- Standard / Gradual Approach, 12 weeks: 5 mL total (1 mL per vial) → 2 × 3 mL vials.
- Standard / Gradual Approach, 24 weeks: 10 mL total (1 mL per vial) → 4 × 3 mL vials.

**Alcohol swabs**
- Standard / Gradual Approach, 12 weeks: 168 minimum; one box of 100 does not cover the calculation.
- Standard / Gradual Approach, 24 weeks: 336 minimum; one box of 100 does not cover the calculation.

## Storage Instructions

- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.

## How This Works

Hypothalamic-pituitary-adrenal axis modulation In research literature, a reduction in nocturnal cortisol release has been documented, associated with deeper sleep in models Increased cerebral adenosine: boost sleep homeostatic pressure Neurotransmitter Regulation balances acetylcholine, GABA, and glutamate in patterns favorable to deep sleep Endogenous antioxidant reduce neuronal oxidative stress accumulated during wakefulness Without direct action on melatonin or benzodiazepine receptors: Mechanism other than pharmaceutical hypnotics

## References

1. Schoenenberger GA, Monnier M. Characterization of a delta-electroencephalogram (-sleep)-inducing peptide. Proc Natl Acad Sci USA. 1977;74(3):1282-1286.
2. Graf MV, Kastin AJ. Delta-sleep-inducing peptide (DSIP): a review. Neurosci Biobehav Rev. 1984;8(1):83-93.
3. Sudakov KV, Ivanov VT, Koplik EV, et al. Delta-sleep inducing peptide (DSIP) as a factor facilitating animals’ resistance to acute emotional stress. Pavlov J Biol Sci. 1983;18(1):1-5.

---
Source: https://protocolopeptidos.com/en/compuestos/dsip-5mg · Important: this guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.