AHK-Cu Quick Reference (100 mg vial)
Research context: mechanisms, human and preclinical evidence, limitations and precautions are in How this works and References. The calculator (Spanish) converts any other volume.
AHK-Cu Dosage Chart (100 mg vial)
No published clinical trial establishes a dosing regimen for AHK-Cu. Any figure circulating as a human protocol does not come from an indexed trial. This site does not publish doses without a verifiable source.
U-100 Conversion Table (3 mL = 33.33 mg/mL)
| U-100 units | Volume | Contains | Yield per vial |
|---|---|---|---|
| 2 | 0.02 mL | 666.667 mcg | 149 injections |
| 5 | 0.05 mL | 1.66667 mg (1667 mcg) | 59 injections |
| 10 | 0.10 mL | 3.33333 mg (3333 mcg) | 29 injections |
| 20 | 0.20 mL | 6.66667 mg (6667 mcg) | 14 injections |
| 30 | 0.30 mL | 10 mg (10000 mcg) | 9 injections |
| 50 | 0.50 mL | 16.6667 mg (16667 mcg) | 6 injections |
| 100 | 1.00 mL | 33.3333 mg (33333 mcg) | 3 injections |
Reconstitution Steps
- Draw 3 mL of bacteriostatic water with a sterile syringe.1
- Inject slowly down the vial wall; avoid shaking to prevent foaming.2
- Gently swirl or roll until fully dissolved (clear solution).3
- Label with the reconstitution date and refrigerate at 2–8 °C, protected from light; use within 4 weeks.4
Supplies Needed
Per 100 mg vial: 3 mL of bacteriostatic water, one U-100 syringe per injection (plus one to reconstitute) and two alcohol swabs per injection.
AHK-Cu 100mgView in store
Bacteriostatic water 3 mLView in storeStore links may earn a commission. A supplier page is not scientific evidence or proof of suitability for human use.
AHK-Cu Vial and Research Context
- Reconstitute: add 3 mL of bacteriostatic water to produce 33.33 mg/mL.
- Easy measuring: 1 U-100 unit = 0.01 mL = 333 mcg.
- Vial size: the 100 mg label is total nominal content, not a per-injection amount.
Storage Instructions
- Lyophilized (unopened): −20 °C for long-term storage; 2–8 °C if used within weeks. Protected from light.
- Reconstituted: 2–8 °C, do not freeze. Use within the documented period (typically up to 4 weeks with bacteriostatic water).
- Handling: do not shake; avoid temperature cycling; label with date and concentration.
- Discard: cloudy solutions, particulates or expired preparations are discarded.
More detail in the storage guide (Spanish).
Important Notes
- Research only: the referenced compounds are sold for laboratory research. Nothing here is medical advice or an administration schedule.
- Above 100 units: a 1 mL U-100 syringe cannot hold them; split across syringes or reconstitute with less volume.
- Accuracy: concentration depends on the actual volume added; measure the diluent with a syringe, not by eye.
- Verification: confirm vial contents against the batch certificate of analysis before calculating.
How This Works
AHK-Cu is the synthetic tripeptide Ala-His-Lys (Alanyl-Histidyl-Lysine) complexed with copper (II), presented as a 100 mg lyophilized research vial. The literature documents it in hair follicle and dermal matrix studies as a copper complex associated with cell proliferation signaling.
Signaling pathways documented in the literature:
- Follicle stimulation Ex vivo models of human hair follicles document elongation and proliferation of dermal papilla cells
- Copper Signaling: The copper (II) complex is associated in literature with extracellular matrix modulation and dermal angiogenesis
Research Applications
- Pyo HK et al., Biol Pharm Bull 2007 (PMID 17703734): AHK-Cu in in vitro human hair follicle growth and dermal papilla cell proliferation studies
- Pickart L, Margolina A, Int J Mol Sci 2018 (PMID 30041448): Copper peptide review in dermal remodeling and extracellular matrix
Research material only. No FDA-approved human use. Information is provided for educational and scientific literature reference purposes.
Injection Technique in Research
- Route in the studies: subcutaneous, in abdominal or thigh adipose tissue, rotating the site.
- Syringe: 1 mL U-100 (100 units); read in units, not mL.
- Asepsis: alcohol swab on the stopper and the site; a new syringe per injection.
- Drawing: purge air, draw the units from the chart and verify before withdrawing the needle from the vial.
Full guide: U-100 syringes and reading units (Spanish).
Research Note
All content on this page is technical and educational. It describes how a vial is reconstituted, how a syringe is read and which amounts the cited sources describe. It does not replace the supervision of a qualified researcher or the judgment of a health professional, and it is not intended to diagnose, treat, cure or prevent any disease.